Jonathan Clarhaut

Publications HAL

Julie Charles, Jonathan Clarhaut, Laure Prouvensier, Tom Collet, Lucas Keomany, et al.. Assessment of in vitro efficacy of three-antibiotics combinations against Mycobacterium abscessus. ESCMID Global 2025, Apr 2025, Vienna, Austria. . ⟨hal-05071423⟩
Arnaud Monvoisin, C. Simmoneau, Jonathan Clarhaut, A Minaoui, Norah Defamie, et al.. Role of gap junctional intercellular communication in lymphangiogenesis and in prostate cancer cell adhesion to lymphatic endothelium. 18th International Vascular Biology Meeting, 2014, Kyoto, Japan. ⟨hal-02498248⟩

Guillermo Landa, Jonathan Clarhaut, Julien Buyck, Gracia Mendoza, Manuel Arruebo, et al.. Impact of mixed Staphylococcus aureus-Pseudomonas aeruginosa biofilm on susceptibility to antimicrobial treatments in a 3D in vitro model. Scientific Reports, 2024, 14 (1), pp.27877. ⟨10.1038/s41598-024-79573-y⟩. ⟨hal-05144149⟩
Jérémy Moreau, Thierry Noël, Kévin Point, Frederic Tewes, Luc Deroche, et al.. Pan-azole-resistant Meyerozyma guilliermondii clonal isolates harbouring a double F126L and L505F mutation in Erg11.. Mycoses, 2024, 67 (3), pp.e13704. ⟨10.1111/myc.13704⟩. ⟨hal-04715648⟩
Elodie Péraudeau, Brigitte Renoux, Sheik Emambux, Pauline Poinot, Rémi Châtre, et al.. Combination of Targeted Therapies for Colorectal Cancer Treatment. Molecular Pharmaceutics, 2023, 20 (9), pp.4537-4545. ⟨10.1021/acs.molpharmaceut.3c00224⟩. ⟨hal-05019569⟩
Elodie Péraudeau, Brigitte Renoux, Sheik Emambux, Pauline Poinot, Rémi Châtre, et al.. Combination of Targeted Therapies for Colorectal Cancer Treatment. Molecular Pharmaceutics, 2023, 20 (9), pp.4537-4545. ⟨10.1021/acs.molpharmaceut.3c00224⟩. ⟨hal-04508239⟩
Rémi Châtre, Estelle Blochouse, Rony Eid, Fabiola Djago, Justin Lange, et al.. Induced-volatolomics for the design of tumour activated therapy. Chemical Science, 2023, 14 (18), pp.4697-4703. ⟨10.1039/d2sc06797h⟩. ⟨hal-04098727⟩
Alexia Chauzy, Salim Bouchène, Vincent Aranzana-Climent, Jonathan Clarhaut, Christophe Adier, et al.. A Minimal Physiologically Based Pharmacokinetic Model to Characterize CNS Distribution of Metronidazole in Neuro Care ICU Patients. Antibiotics, 2022, 11 (10), pp.1293. ⟨10.3390/antibiotics11101293⟩. ⟨hal-03906712⟩
Chad Plumet, Rémi Châtre, Fabiola Djago, Elodie Péraudeau, Quentin Blancart-Remaury, et al.. A β-Cyclodextrin-Albumin Conjugate for Enhancing Therapeutic Efficacy of Cytotoxic Drugs. Bioconjugate Chemistry, 2022, 33 (6), pp.1138-1144. ⟨10.1021/acs.bioconjchem.2c00182⟩. ⟨hal-03765739⟩
Chad Plumet, Achmet Said Mohamed, Tanguy Vendeuvre, Brigitte Renoux, Jonathan Clarhaut, et al.. Cell–cell interactions via non-covalent click chemistry. Chemical Science, 2021, 12 (26), pp.9017-9021. ⟨10.1039/D1SC01637G⟩. ⟨hal-03285569⟩
Chad Plumet, Achmet Said Mohamed, Tanguy Vendeuvre, Brigitte Renoux, Jonathan Clarhaut, et al.. Cell–cell interactions via non-covalent click chemistry. Chemical Science, 2021, 12 (26), pp.9017-9021. ⟨10.1039/D1SC01637G⟩. ⟨hal-05019654⟩
Rémi Châtre, Justin Lange, Elodie Péraudeau, Pauline Poinot, Stéphanie Lerondel, et al.. In vivo synthesis of triple-loaded albumin conjugate for efficient targeted cancer chemotherapy. Journal of Controlled Release, 2020, 327, pp.19-25. ⟨10.1016/j.jconrel.2020.08.008⟩. ⟨hal-03491375⟩
Somia Haouache, Ayman Karam, Tony Chave, Jonathan Clarhaut, Prince Nana Amaniampong, et al.. Selective radical depolymerization of cellulose to glucose induced by high frequency ultrasound. Chemical Science, 2020, 11 (10), pp.2664-2669. ⟨10.1039/D0SC00020E⟩. ⟨hal-02921911⟩
Jonathan Boucher, Annie-Claire Balandre, Marjolaine Debant, Justine Vix, Thomas Harnois, et al.. Cx43 Present at the Leading Edge Membrane Governs Promigratory Effects of Osteoblast-Conditioned Medium on Human Prostate Cancer Cells in the Context of Bone Metastasis. Cancers, 2020, 12 (10), pp.3013. ⟨10.3390/cancers12103013⟩. ⟨hal-03176921⟩
Justin Lange, Balkis Eddhif, Mehrad Tarighi, Théa Garandeau, Elodie Péraudeau, et al.. Volatile Organic Compound Based Probe for Induced Volatolomics of Cancers. Angewandte Chemie International Edition, 2019, 58 (49), pp.17563-17566. ⟨10.1002/anie.201906261⟩. ⟨hal-04508274⟩
Karine Porte, Brigitte Renoux, Elodie Péraudeau, Jonathan A. Clarhaut, Balkis Eddhif, et al.. Controlled Release of a Micelle Payload via Sequential Enzymatic and Bioorthogonal Reactions in Living Systems. Angewandte Chemie International Edition, 2019, 58 (19), pp.6366-6370. ⟨10.1002/anie.201902137⟩. ⟨hal-02115783⟩
Balkis Eddhif, Justin Lange, Nadia Guignard, Yann Batonneau, Jonathan A. Clarhaut, et al.. 2-Acetamido-2-deoxy- l -iminosugar C -Alkyl and C -Aryl Glycosides: Synthesis and Glycosidase Inhibition. European Journal of Organic Chemistry, 2018, 2018 (40), pp.5477-5488. ⟨10.1002/ejoc.201800678⟩. ⟨hal-02172201⟩
Guillaume Compain, Nassima Oumata, Jonathan A. Clarhaut, Elodie Péraudeau, Brigitte Renoux, et al.. A β-glucuronidase-responsive albumin-binding prodrug for potential selective kinase inhibitor-based cancer chemotherapy. European Journal of Medicinal Chemistry, 2018, 158, pp.1-6. ⟨10.1016/j.ejmech.2018.08.100⟩. ⟨hal-02169782⟩
Jonathan Boucher, Claire Simonneau, Golthlay Denet, Jonathan Clarhaut, Claire Balandre, et al.. Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis. International Journal of Molecular Sciences, 2018, 19 (6), pp.1558. ⟨10.3390/ijms19061558⟩. ⟨hal-02129345⟩
Balkis Eddhif, Nadia Guignard, Yann Batonneau, Jonathan A. Clarhaut, Sébastien Papot, et al.. TCA precipitation and ethanol/HCl single-step purification evaluation: One-dimensional gel electrophoresis, bradford assays, spectrofluorometry and Raman spectroscopy data on HSA, Rnase, lysozyme - Mascots and Skyline data. Data in Brief, 2018, 17, pp.938-953. ⟨10.1016/j.dib.2018.01.095⟩. ⟨hal-02169942⟩
Claude Geffroy-Rodier, Balkis Eddhif, Justin Lange, Nadia Guignard, Yann Batonneau, et al.. Study of a novel agent for TCA precipitated proteins washing - comprehensive insights into the role of ethanol/HCl on molten globule state by multi-spectroscopic analyses. Journal of Proteomics, 2018, 173, pp.77 - 88. ⟨10.1016/j.jprot.2017.11.016⟩. ⟨insu-01823781⟩
Brigitte Renoux, Laure Fangous, Camille Hötten, Elodie Péraudeau, Balkis Eddhif, et al.. A β-glucuronidase-responsive albumin-binding prodrug programmed for the double release of monomethyl auristatin E. MedChemComm, 2018, 9 (12), pp.2068-2071. ⟨10.1039/C8MD00466H⟩. ⟨hal-04508272⟩
E. Peraudeau, L. Cronier, A. Monvoisin, P. Poinot, C. Mergault, et al.. Enhancing tumor response to targeted chemotherapy through up-regulation of folate receptor α expression induced by dexamethasone and valproic acid. Journal of Controlled Release, 2018, 269, pp.36-44. ⟨10.1016/j.jconrel.2017.11.011⟩. ⟨hal-02122599⟩
Brigitte Renoux, Laure Fangous, Camille Hötten, Élodie Péraudeau, Balkis Eddhif, et al.. A β-glucuronidase-responsive albumin-binding prodrug programmed for the double release of monomethyl auristatin E. MedChemComm, 2018, 9 (12), pp.2068-2071. ⟨10.1039/C8MD00466H⟩. ⟨hal-05038390⟩
Sergii Kolodych, Chloé Michel, Sébastien Delacroix, Oleksandr Koniev, Anthony Ehkirch, et al.. In situ targeted activation of an anticancer agent using ultrasound-triggered release of composite droplets. European Journal of Medicinal Chemistry, 2017, 142, pp.2-7. ⟨10.1016/j.ejmech.2017.03.057⟩. ⟨hal-02174984⟩
Marine Bezagu, Jonathan Clarhaut, Brigitte Renoux, Fabrice Monti, Mickael Tanter, et al.. In situ targeted activation of an anticancer agent using ultrasound-triggered release of composite droplets. European Journal of Medicinal Chemistry, 2017, 142, pp.2-7. ⟨10.1016/j.ejmech.2017.03.057⟩. ⟨hal-04088862⟩
Brigitte Renoux, Florian Raes, Thibaut Legigan, Elodie Péraudeau, Balkis Eddhif, et al.. Targeting the tumour microenvironment with an enzyme-responsive drug delivery system for the efficient therapy of breast and pancreatic cancers. Chemical Science, 2017, 8 (5), pp.3427-3433. ⟨10.1039/C7SC00472A⟩. ⟨hal-02174996⟩
S. Rubio, J. Clarhaut, E. Peraudeau, M. Vincenzi, C. Soum, et al.. Diminished oligomerization in the synthesis of new anti-angiogenic cyclic peptide using solution instead of solid-phase cyclization. Biopolymers, 2016, pp.368-375. ⟨10.1002/bip.22814⟩. ⟨hal-01563735⟩
Jérôme Alsarraf, Elodie Peraudeau, Pauline Poinot, I. Tranoy-Opalinski, Jonathan Clarhaut, et al.. A dendritic β-galactosidase-responsive folate–monomethylauristatin E conjugate. Chemical Communications, 2015, 51 (87), pp.15792-15795. ⟨10.1039/C5CC05294G⟩. ⟨hal-03127679⟩
Denis Sarrouilhe, Jonathan Clarhaut, Norah Defamie, Marc Mesnil. Serotonin and Cancer: What Is the Link?. Current Molecular Medicine, 2015, 15 (1), pp.62-77. ⟨10.2174/1566524015666150114113411⟩. ⟨hal-04432521⟩
Romain Barat, Thibaut Legigan, I. Tranoy-Opalinski, Brigitte Renoux, Elodie Peraudeau, et al.. A mechanically interlocked molecular system programmed for the delivery of an anticancer drug. Chemical Science, 2015, 6 (4), pp.2608-2613. ⟨10.1039/c5sc00648a⟩. ⟨hal-03127729⟩
Isabelle Tranoy-Opalinski, Thibaut Legigan, Romain Barat, Jonathan Clarhaut, Mikael Thomas, et al.. beta-Glucuronidase-responsive prodrugs for selective cancer chemotherapy: An update. European Journal of Medicinal Chemistry, 2014, 74, pp.302-313. ⟨10.1016/j.ejmech2013.12.045⟩. ⟨hal-01196935⟩
Laurie-Anne Payet, Ludovic Pineau, Ellen C R Snyder, Jenny Colas, Ahmed Moussa, et al.. Saturated fatty acids alter the late secretory pathway by modulating membrane properties.. Traffic, 2013, 14 (12), pp.1228-41. ⟨10.1111/tra.12117⟩. ⟨hal-00990166⟩
M. Grinda, T. Legigan, J. Clarhaut, E. Peraudeau, I. Tranoy-Opalinski, et al.. An enzyme-responsive system programmed for the double release of bioactive molecules through an intracellular chemical amplification process. Organic & Biomolecular Chemistry, 2013, 11 (41), pp.7129-7133. ⟨10.1039/c3ob41536h⟩. ⟨hal-00966980⟩
Thibaut Legigan, Jonathan Clarhaut, Brigitte Renoux, Isabelle Tranoy-Opalinski, Arnaud Monvoisin, et al.. Synthesis and biological evaluations of a monomethylauristatin E glucuronide prodrug for selective cancer chemotherapy. European Journal of Medicinal Chemistry, 2013, 67, pp.75-80. ⟨10.1016/j.ejmech.2013.06.037⟩. ⟨hal-04033523⟩
Jonathan Clarhaut, Sylvain Fraineau, Joëlle Guilhot, Elodie Péraudeau, Isabelle Tranoy-Opalinski, et al.. A galactosidase-responsive doxorubicin-folate conjugate for selective targeting of acute myelogenous leukemia blasts. Leukemia Research, 2013, 37 (8), pp.948 - 955. ⟨10.1016/j.leukres.2013.04.026⟩. ⟨inserm-02444614⟩
Marina Yefimova, Nadia Messaddeq, Thomas Harnois, Annie-Claire Meunier, Jonathan Clarhaut, et al.. A chimerical phagocytosis model reveals the recruitment by Sertoli cells of autophagy for the degradation of ingested illegitimate substrates. Autophagy, 2013, 9 (5), pp.653-666. ⟨10.4161/auto.23839⟩. ⟨hal-03119928⟩
Sylvain Fraineau, Arnaud Monvoisin, Jonathan Clarhaut, Julie Talbot, Claire Simonneau, et al.. The vitamin K–dependent anticoagulant factor, protein S, inhibits multiple VEGF-A–induced angiogenesis events in a Mer- and SHP2-dependent manner. Blood, 2012, 120 (25), pp.5073-5083. ⟨10.1182/blood-2012-05-429183⟩. ⟨inserm-02444598⟩
Pierre-Olivier Strale, Jonathan Clarhaut, Coralie Lamiche, Laurent Cronier, Marc Mesnil, et al.. Down-regulation of Connexin43 expression reveals the involvement of caveolin-1 containing lipid rafts in human U251 glioblastoma cell invasion.. Molecular Carcinogenesis, 2012, 51 (11), pp.845-60. ⟨10.1002/mc.20853⟩. ⟨hal-00993046⟩
Thibaut Legigan, Jonathan Clarhaut, Isabelle Tranoy-Opalinski, Arnaud Monvoisin, Brigitte Renoux, et al.. The First Generation of β-Galactosidase-Responsive Prodrugs Designed for the Selective Treatment of Solid Tumors in Prodrug Monotherapy. Angewandte Chemie International Edition, 2012, 51, pp.1 - 6. ⟨10.1002/anie.201204935⟩. ⟨hal-00736090⟩
Thibaut Legigan, Jonathan Clarhaut, Brigitte Renoux, Isabelle Tranoy-Opalinski, Arnaud Monvoisin, et al.. Synthesis and Antitumor Efficacy of a β-Glucuronidase-Responsive Albumin-Binding Prodrug of Doxorubicin.. Journal of Medicinal Chemistry, 2012, 55 (9), pp.4516-20. ⟨10.1021/jm300348r⟩. ⟨hal-00710692⟩
Coralie Lamiche, Jonathan Clarhaut, Pierre-Olivier Strale, Sophie Crespin, Nathalie Pedretti, et al.. The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells.. Clinical and Experimental Metastasis, 2012, 29 (2), pp.111-22. ⟨10.1007/s10585-011-9434-4⟩. ⟨hal-00993073⟩
Marion Grinda, J. Clarhaut, Brigitte Renoux, Isabelle Tranoy-Opalinski, Sébastien Papot. A self-immolative dendritic glucuronide prodrug of doxorubicin. MedChemComm, 2012, 3 (1), pp.68-70. ⟨10.1039/c1md00193k⟩. ⟨hal-00682705⟩
Marion Grinda, Jonathan Clarhaut, Isabelle Tranoy-Opalinski, Brigitte Renoux, Arnaud Monvoisin, et al.. A Heterodimeric Glucuronide Prodrug for Cancer Tritherapy: the Double Role of the Chemical Amplifier. ChemMedChem, 2011, 6 (12), pp.2137-2141. ⟨10.1002/cmdc.201100355⟩. ⟨hal-04033576⟩
Graziello Geneau, Coralie Lamiche, Corinne Niger, Pierre-Olivier Strale, Jonathan A. Clarhaut, et al.. Effect of endothelin-1 on osteoblastic differentiation is modified by the level of connexin43: comparative study on calvarial osteoblastic cells isolated from Cx43+/- and Cx43+/+ mice.. Cell and Tissue Research, 2010, 340 (1), pp.103-15. ⟨10.1007/s00441-009-0924-5⟩. ⟨hal-00498245⟩
Jonathan A. Clarhaut, Robert M. Gemmill, Vincent A. Potiron, Slimane Ait-Si-Ali, Jean Imbert, et al.. ZEB-1, a repressor of the semaphorin 3F tumor suppressor gene in lung cancer cells.. Neoplasia, 2009, 11 (2), pp.157-166. ⟨10.1593/neo.81074⟩. ⟨hal-00416521⟩
Cédric Charrier, Jonathan A. Clarhaut, Jean-Pierre Gesson, Guillermina Estiu, Olaf Wiest, et al.. Synthesis and Modeling of New Benzofuranone Histone Deacetylase Inhibitors that Stimulate Tumor Suppressor Gene Expression. Journal of Medicinal Chemistry, 2009, 52, pp.3112-3115. ⟨hal-00438795⟩
Mickaël Thomas, Jonathan A. Clarhaut, Isabelle Tranoy-Opalinski, Jean-Pierre Gesson, Joëlle Roche, et al.. Synthesis and biological evaluation of glucuronide prodrugs of the histone deacetylase inhibitor CI-994 for application in selective cancer chemotherapy.. Bioorganic and Medicinal Chemistry, 2008, 16 (17), pp.8109-8116. ⟨10.1016/j.bmc.2008.07.048⟩. ⟨hal-00458881⟩
Lucie Karayan-Tapon, Michel Wager, Joëlle Guilhot, Pierre Levillain, C. Marquant, et al.. Semaphorin, neuropilin and VEGF expression in glial tumours: SEMA3G, a prognostic marker?. British Journal of Cancer, 2008, 99 (7), pp.1153-1160. ⟨10.1038/sj.bjc.6604641⟩. ⟨hal-00458867⟩
Vincent A. Potiron, Girish Sharma, Patrick Nasarre, Jonathan A. Clarhaut, Hellmut G. Augustin, et al.. Semaphorin SEMA3F affects multiple signaling pathways in lung cancer cells. Cancer Research, 2007, 67 (18), pp.8708-8715. ⟨10.1158/0008-5472.CAN-06-3612⟩. ⟨hal-00416414⟩
J. Clarhaut, J. Roche, H.A. Drabkin. semaphorins in lung cancer. Journal of Thoracic Oncology, 2006, 1, pp.203-204. ⟨hal-00078139⟩

Communication dans un congrès (1 document)

Kévin Brunet, Jonathan Clarhaut, Adrien Baillod, Sandrine Marchand, Frederic Tewes. Enhancement of amphotericin B activity against Cryptococcus neoformans using lipid nanoparticles. 33rd ECCMID : European Congress of Clinical Microbiology & Infectious Diseases, Apr 2023, Copenaghen, Denmark. ⟨hal-04142666⟩

Short CV:

Dr. Jonathan Clarhaut (born 04.06.1982, France) is an PhD-HDR-Engineer in cell biology at the CHU of Poitiers. He has been hired in 2012 to work on anticancer vectorization strategies, before joining INSERM U1070 in 2022 to develop the cell culture department. With more than 49 peer-reviewed publications, Jonathan Clarhaut is a specialist in the study of drug interactions with eukaryotic cells. Since December 2024, he has been Administrative Director, responsible for the unit’s administrative and budgetary coordination.

Teaching:

60h/year

– « Stabilité et Variabilité du Génome »

– « Méthodologie en Génie Génétique »

– « Génétique et immunologie »

Technical skills:

Cell biology: Cell culture of adherent and non-adherent cell lines, primary cell culture of endothelial cells, monolayer cell barrier model, transient transfection, RNA interference (siRNA), angiogenic assay (morphogenesis on Matrigel), viability/cytotoxicity assay (XTT), proliferation assay and (BrdU cell cycle incorporation : ELISA, FACs), migration/invasion tests (Boyden chamber), phenotypic analysis of membrane markers by FACS, apoptosis test (Annexin V by FACs), growth test without matrix anchoring and fluorescence imaging under dynamic flow.

Biochemistry and molecular biology: RNA extraction and purification, reverse transcription (RT) and real-time quantitative PCR (SYBR Green), digital PCR (dPCR) PCR, protein and histone extractions, chromatin immunoprecipitation (ChIP), Sanger DNA sequencing, Western blotting, immunoprecipitation, zymography, immunocytochemistry, immunohistochemistry.

Microbiology: Growth curve, Minimum Inhibitory Concentration (MIC)

Main recent publications:

Accès directs

Menu principal

Jonathan Clarhaut

Publications HAL