Microbiology and molecular biology
Supervisor: Dr. J. Buyck
In vitro pharmacoynamic evaluation of anti-infectious agents
- Technics used
Antibiotic resistance assessments: MIC, checkerboard, Time-kill curves, Hollow fiber
MIC and checkerboard on fungi and mucorales
40 m² BSL2 lab
3 PSMII, equipped for in vitro experiments
CO2 incubator for in vitro intracellular model of infections (Pseudomonas aeruginosa, Mycobacterium abscessus
Automated plating and counting of CFU
Supervisor: Dr J. Buyck.
Characterization of resistance mechanisms
- Quantification of gene expression: Resistance Gene expression analysis is fundamental to understand dynamic changes in a bacterium in the contact of antibiotic. We have developed the gene expression analysis by qRTPCR on many genes involved in the resistance development in various species as: Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii.
- PCR and Sequencing: Amplification sequencing of DNA fragments allows to study the identification of mutations conferring for exemple resistance phenotypes during antibiotic exposure. We are regularly sequencing gene involvein the resistance of antibiotics to various species as Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii
- Access to WGS/NGS platform
- Site-Directed Mutagenesis: Construction of bacterial gene-inactivating plasmids, Construction of mutants (Knock out, Knock In, Mutational insertion) in Enterobacteriaceae, A. Baumannii and aeruginosa even on MDR clinical isolates.
- Construction of reporter gene: It allows to follow expression of a gene of interest by fluorescence or luminescence